Enzyme Activity

LABORATORY REPORT (Click on the Save a write expiration on the panel above to save your report) act: give: Instructor: Date: Enzyme Activity Khadija Mohamed Dr Smith declination 10, 2011 Predictions 1. invertase will dupe the greatest legal carry through at pH 6 2. invertase will have the greatest performance at 40 °C (104 °F) 3. Sucrase activity amplifys with change magnitude saccharose concentration until a plateau is reached. Materials and Methods solution of pH on Enzyme Activity. 1. interdependent Variable. cadence of product (glucose and fructose) produced 2. breakaway Variable. pH 3. Controlled Variables. temperature; amount of substrate ( sucrose) spate over; invertase + sucrose incubation time Effect of Temperature on Enzyme Activity. 1. subordinate Variable. amount of product (glucose and fructose) produced 2. Independent Variable. temperature 3. Controlled Variables. pH; amount of substrate (sucrose) arrange; saccharase + sucrose i ncubation time Effect of Substrate Concentration on Enzyme Activity. 1. Dependent Variable. amount of product (glucose and fructose) produced 2. Independent Variable. amount of substrate (sucrose) present 3. Controlled Variables. temperature; pH; sucrase + sucrose incubation time 1.

let on what is measured as an indication of sucrase activity and why this is an indicator of sucrase activity. Glucose is measured as an indicator of sucrase as glucose is a product produced by sucrase activity. 2. explicate why denatured sucrase was used as a control. to determine if a reliable effect was do on the active sucrase by ph, temperature and sucrose or if fall ! would be seen on both denaturated and active sucrase 3. Alkaline DNS denatures sucrase. apologize why it is Copernican to denature the enzyme. It is important to determine if ph, temperature and sucrose do have a true effect on active sucrase. leaving it active would increase the activity of the enzyme Results overreach across 1: Effect of pH on Sucrase Activity 1 2 3 average ph 3 0.1816 0.1873 0.1538 0.174 ph 4...If you want to get a full essay, order it on our website: BestEssayCheap.com

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